A Investigating The Effect of Insect Body Extracts (Their Products) and Some Arthropods in Inhibiting The Growth of Cancer Cells or Tumours (Gastric cancer cell line/AGS) Investigating the Effect of Insect Body Extracts and Arthropods in Inhibiting the Growth of Gastric Cancer Cells (AGS)
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Gastric cancer, Entomotherapy, extract, cancer cells, AGS.
Abstract
Background and purpose: Arthropods including insects and Chilopoda and their products were used as medicine in ancient civilizations. Cancer is a burning disease that is one of the great problems of medical science, and no medicine has been discovered to control and suppress it. This research has investigated the effect of freeze-dried bodies and whole-body contents of some arthropods in inducing apoptosis and death of cancer cells (gastric cancer cell type AGS).
Investigation method: 6 species of insects: Gryllotalpa sp., Polyrhachis sp., Dolichovespula sp., Apis melifera, Periplaneta americana, Drosophila melanogaster and 1 species of Chilopoda Scolopendra sp. These were selected as samples. The samples were washed with distilled water and 70% alcohol, placed in a separate container, frozen and turned into powder. Then the powders were dissolved in 1% DMSO and in eight concentrations of 62.5, 125, 250, 500, 1000, 2000, 4000, and 8000 μg/ml to control the AGS gastric cancer cell line in 6 replicates and 3 separate experiments. It was evaluated by the MTT test. IC50 and LD50 were calculated by Prism version 6 software to check the effect of concentrations determine the effective dose and choose the best extract to control the growth of AGS gastric cancer cells.
Results: (Pol) Polyrhachis sp. It had the best effect in controlling the AGS gastric cancer cell line.
Selected samples after Polyrhachis sp. They had a significant effect on the AGS cancer cell line, respectively: Scolopendra sp., Dolichovespula sp., Apis melifera, Gryllotalpa sp., Peripelaneta Americana, and finally Drosophila melanogaster had the least effect in inducing apoptosis and cell death in the cell line. Cancer cells had AGS, even at a concentration of 8000 micrograms/ml; the cell death did not reach 50% compared to the control. So, more tests can be done for the above samples.
Conclusion: The results of the application of 7 different species of arthropods and insects showed that these species have excellent therapeutic potential, especially in the treatment of AGS cancer cells.
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References
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