Genetic properties of bla CTX-M, bla SHV and bla TEM genes in ESBL producing E. coli clinical isolates
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Submitted
January 3, 2021
Published
January 13, 2021
Keywords:
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E.coli, bla CTX-M, bla SHV, bla TEM, ESBL, antibiotic resistance gene
Abstract
Extended-spectrum β-lactamase (ESBL) producing Escherichia coli is a serious global public health problem that requires timely and accurate control of resistant strains. This is the first report on the detection of resistant E. coli isolates in hospitals of Adjara.
We studied the genetic profile of Escherichia coli isolates producing ESBL isolated from biological samples of patients with suspected nosocomial infection in four hospitals in the Adjara region (Georgia). Identification of resistant genes was performed by multiplex PCR and reverse hybridization. Genes of the class ESBL TEM, ESBL SHV, and CTX-M were found in these samples as a result of molecular studies of genes causing genotypic correlation and resistance. Molecular genetic testing of phenotypically resistant strains of penicillin, cephalosporins of the 3rd and 4th generations, and inhibitors revealed a wide range of antibiotic resistance genes with bla CTX-M, bla TEM, bla SHV, and in two samples with genes, of ESBL class bla TEM type mutated genes: bla TEM AS104 E, bla TEM AS 238 G, bla TEM AS238 S genes.
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References
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3. Mahboobeh Nakhaei Moghaddam, Mehrdad Hashemi Beidokhti, SaeidAmel Jamehdar, and Martha Ghahraman . Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction // Iran J Basic Med Sci. -2014 .- Vol.17, N 5.-P. 378–383.
4. Sadegh Ghorbani-Dalini, Mohammad Kargar, Abbas Doosti, PejmanAbbasi, and MeysamSarshar. Molecular Epidemiology of ESBL Genes and Multi-Drug Resistance in Diarrheagenic Escherichia Coli Strains Isolated from Adults in Iran // Iran J Pharm Res. -2015 .- Vol.14, N 4.- P. 1257–1262.
5. Johnson JR, Johnston B, Clabots C, Kuskowski MA, Castanheira M. Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States// Clin Infect Dis - 2010.-Vol. 51, N3.- P.286–294. https://doi.org/10.1086/653932.
6. Sadeequr Rahman, Tariq Ali, Ijaz Ali, Nazir Ahmad Khan, Bo Han, and Jian Gao. The Growing Genetic and Functional Diversity of Extended Spectrum Beta-Lactamases //- 2018.-https://doi.org/10.1155/2018/9519718.
7. Patricia A. Bradford Extended-Spectrum β-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance //ThreatClin Microbiol Rev- 2001 .-Vol. 14, N 4.-P. 933–951.
8. Huletsky A, Knox J R, Levesque R C. Role of Ser-238 and Lys-240 in the hydrolysis of 3rd-generation cephalosporins by SHV-type beta-lactamases probed by site-directed mutagenesis and 3-dimensional modeling // J Biol Chem. -1993.-Vol. 268, N 5.- P. 3690–3697.
9. W.-H. Zhao and Z.-Q. Hu, “Epidemiology and genetics of CTX-M extended-spectrum β-lactamases in Gram-negative bacteria” //Critical Reviews in Microbiology- 2013.-Vol. 39.- P. 79–101.
10. Oteo J, Navarro C, Cercenado E, Delgado-Iribarren A, Wilhelmi I, Orden B, García C, Miguelañez S, Pérez-Vázquez M, García-Cobos S, Aracil B, Bautista V, Campos J. Spread of Escherichia coli strains with high-level cefotaxime and ceftazidime resistance between the community, long-term care facilities, and hospital institutions // J. Clin. Microbiol. – 2006.- Vol. 44, N 7.-P. 2359–2366.
11. Steward CD, Rasheed JK, Hubert SK, Biddle JW, Raney PM, Anderson GJ, Williams PP, Brittain KL, Oliver A, McGowan JE Jr. Tenover FC. Characterization of clinical isolates of Klebsiella pneumoniae from// 2001.-Vol. 39, N 8.-P. 2864–2872.
12. Koiava T. Gonçalves D. Palmeira J. Arobelidze K, Tediashvili M, AkhvledianiL, Ferreira H. Detection of CTX-m genes antibiotics resisitance in Klebsiella pneumonia isolates of hospitals in adjaria(Georgia)// Georgian Med News .-2016 .-Vol. 258. –P .50-54.
13. Ahmed A. Ramadan, Neveen A. Abdelaziz, Magdy A. Amin, Ramy K. Aziz,Novel blaCTX-M variants and genotype-phenotype correlations among clinical isolates of extended-spectrum beta-lactamase-producing Escherichia coli // Published - 2019 .-Vol .9, N 1.-P. 4224.
14. Tadesse, D. A. et al. Antimicrobial drug resistance in Escherichia coli from humans and food animals, United States, 1950-2002 //Emerg. Infect. Dis. -2012.-Vol. 18, N 5.-P. 741–749. https://doi.org/10.3201/eid1805.111153 .
15. Noha A. Hassuna, Ahmed S. Khairalla, Eman M. Farahat, Adel M. Hammad, Medhat Abdel-Fattah. Molecular characterization of Extended-spectrum β lactamase- producing E. coli recovered from community-acquired urinary tract infections in Upper Egypt //Published .- 2020 .-Vol. 10, N 1.- P. 2772
Authors
Akhvlediani, L. (2021). Genetic properties of bla CTX-M, bla SHV and bla TEM genes in ESBL producing E. coli clinical isolates. Journal of Medical Care Research and Review, 4(1), 480–485. https://doi.org/10.15520/mcrr.v4i1.157
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